CHO 2.0 Media

Next generation basal medium

Next generation feed media

Get full control over protein glycosylation

Best choice for biosimilar protein production

Single cell cloning medium

CHO 2.0 medium documents

CHO 2.0 is a high-performance basal medium engineered for large-scale recombinant protein production. Our powder formula ensures fast and effortless liquid preparation.

CHO 2.0 basal medium is optimized for industry-standard host lines such CHO K1, S, GS, and DG44. In batch cultures, it reaches 2 x 10^7 cells/mL with high viability through day 8. When used in fed-batch processes, cell concentration exceed 3 x 10^7/mL.

By pairing CHO 2.0 basal medium with our specialized feeds, you gain total control over your protein’s glycan profile. This makes it the premier choice for biosimilar protein manufacturing.

CHO 2.0 basal medium

CHO 2.0 basal medium ordering

CHO 2.0 basal medium application

CHO 2.0 feed media documents

CHO 2.0 basal medium is designed to work in synergy with two specialized supplements: Feed A and Feed B. These feeds contain highly concentrated nutrients, including amino acids, vitamins, glucose, and minerals.

By providing this concentrated nutrient boost, the system increases cell density and extends cell viability during production. This robust environment maximizes productivity and ensures superior performance throughout the entire culture process.

CHO 2.0 feed media

CHO 2.0 feed media ordering

CHO 2.0 feed media application

CHO 2.0 glyco-feed documents

Our CHO 2.0 media system offers three specialized Feed A series designed to precisely titrate antibody galactosylation and mannosylation. These feeds provide varying levels of key substrates to modulate glycosylation enzyme activity. By selecting the appropriate feed, you can reach your specific targets for high mannose, G0, G1, and G2 fractions.

This technology is ideal for fine-tuning the glycosylation profiles of biosimilars. It allows for high-precision adjustments in fed-batch processes without the need for genetic engineering of the host cells.

For optimal performance, these glycosylation-specific feeds should be used in combination with our Feed B and our CHO 2.0 basal medium. Together, they form a culture system that ensures both high productivity and superior product quality.

Titrate glyco profile of your protein

CHO 2.0 glyco-feed ordering

CHO 2.0 glyco-feed application

with CHO 2.0 media system

LILY single cell cloning medium documents

LILY is a ready to use medium, which promotes clonal growth of CHO cells, no matter which cloning method is performed, manually by limited dilution or by automatic cell seeding with a device.

Medium is serum free and animal component free. It contains recombinant proteins.