Metabolites of primary cells can be products like cytokines, growth factors, exosomes or a combination of may such substances as a cocktail. In order to produce such primary cell-based products first the primary cells need to be cultured in large scale and thereafter the particular product need to be isolated from supernatant.
Primary cells have special requirement to culture media because of their dependency to growth factors and different metabolism compared to continuous cell lines. Florabio design special culture media for primary cells fulfilling their nutritional requirement. Proprietary culture media are developed and produced for large scale cultivation of primary cells.
Further, primary cells grow often adherently and adaptation to suspension growth conditions is difficult or impossible without losing their cellular properties. Florabio design production processes in small scale considering cell growth requirements, e.g. on micro carrier or in fixed-bed cultivation methodology.
Scale up of primary cell culture processes can be challenging as well due to the share sensitivity and adherent growth properties. Florabio perform the large scale and thus make it possible to produce primary cell-based products in large scale. Isolation, concentration and stabilisation of the product is the last process step which will be performed by Florabio in small as well as in large scale.